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Bovine Brucella Ab ELISA Kit
65 USD
Category:Kontrol ve analiz cihazları ve ekipmanları
Bovine brucellosis is a highly contagious bacterial disease, almost exclusively caused by Brucella abortus causing late term-abortion and infertility in cattle. The disease is also a serious zoonosis, causing undulant fever in humans.
The BIONOTE B. Brucella Ab ELISA 2.0 is an indirect Enzyme Linked Immunosorbent Assay(ELISA) for the qualitative detection of Brucella abortus antibody in serum, plasma or milk.
The BIONOTE B. Brucella Ab ELISA 2.0 contains a microplate, which is pre-coated with non-infectious Brucella antigen on the well. During first incubation, Brucella antibodies(if present in the test sample) bind to the antigens in the well. Unbound materials are removed by aspiration and washing. HRP conjugate added subsequently forms a complex with the Brucella antibodies. Unbound materials are removed by aspiration and washing again and substrate solution is added. The residual enzyme activity found in the well will thus be directly proportional to the Brucella antibody concentration in samples. The reaction is stopped by addition of the stopping solution and colorimetric reading should be performed by using a spectrophotometer at 450 ㎚with reference wavelength at 620 ㎚.
65 USD
